We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Sweetener System Intervention Shifted Neutrophils from Homeostasis to Priming.
- Authors
Skurk, Thomas; Krämer, Tamara; Marcinek, Patrick; Malki, Agne; Lang, Roman; Dunkel, Andreas; Krautwurst, Tiffany; Hofmann, Thomas F.; Krautwurst, Dietmar
- Abstract
Background: Non-nutritive sweeteners (NNS) are part of personalized nutrition strategies supporting healthy glycemic control. In contrast, the consumption of non-nutritive sweeteners has been related to person-specific and microbiome-dependent glycemic impairments. Reports on the effects of NNS on our highly individual cellular immune system are sparse. The recent identification of taste receptor expression in a variety of immune cells, however, suggested their immune-modulatory relevance. Methods: We studied the influence of a beverage-typical NNS system on the transcriptional profiling of sweetener-cognate taste receptors, selected cytokines and their receptors, and on Ca2+ signaling in isolated blood neutrophils. We determined plasma concentrations of saccharin, acesulfame-K, and cyclamate by HPLC-MS/MS, upon ingestion of a soft drink-typical sweetener surrogate. In an open-labeled, randomized intervention study, we determined pre- versus post-intervention transcript levels by RT-qPCR of sweetener-cognate taste receptors and immune factors. Results: Here we show that the consumption of a food-typical sweetener system modulated the gene expression of cognate taste receptors and induced the transcriptional regulation signatures of early homeostasis- and late receptor/signaling- and inflammation-related genes in blood neutrophils, shifting their transcriptional profile from homeostasis to priming. Notably, sweeteners at postprandial plasma concentrations facilitated fMLF (N-formyl-Met-Leu-Phe)-induced Ca2+ signaling. Conclusions: Our results support the notion of sweeteners priming neutrophils to higher alertness towards their adequate stimuli.
- Subjects
HOMEOSTASIS; CYTOKINES; REVERSE transcriptase polymerase chain reaction; GLYCEMIC control; FOOD consumption; BLOOD plasma; SACCHARIN; SWEETENERS; NEUTROPHILS; CELLULAR signal transduction; GENE expression; HUMAN microbiota; RESEARCH funding; CARBONATED beverages; TRANSCRIPTION factors; CYCLAMATES
- Publication
Nutrients, 2023, Vol 15, Issue 5, p1260
- ISSN
2072-6643
- Publication type
Article
- DOI
10.3390/nu15051260