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- Title
Antimicrobial Activity of a Formulation for the Low Temperature Disinfection of Critical and Semi-critical Medical Equipment and Surfaces.
- Authors
Raffo, Paolo; Salliez, A. C.; Collignon, Christian; Clementi, Massimo
- Abstract
Aims: The antimicrobial activity of the disinfectant formulation isopropyl-tridecyl-dimethyl- ammonium (UMONIUM38: Huckert's International, Nivelles, Belgium) planned for the low temperature disinfection of critical and semi-critical medical equipment and surfaces was evaluated under clean and dirty experimental conditions (high and low concentrations of organic material). Material & methods: The formulation was obtained by a synergic combination of three different active compounds, two alcohols and a quaternary ammonium. The anti-mycobacterial (Mycobacterium avium and Mycobacterium terrae), and antiviral (Poliovirus type 1, Adenovirus type 5, hepatitis B virus, and human immunodeficiency virus type 1) activities of this formulation were addressed using suspension assays. In addition, surface assays were also used to test the antibacterial (Pseudomonas aeruginosa, Escherichia coil, Staphylococcus aureus, Enterococcus hirae) and antimycotic (Canclida albicans and Aspergillus niger) activities. Results: Using standardised methods, a reduction of the virus titers higher than 4 Log10 required to document the virucidal activity of a disinfectant formulation was obtained using 2.5% isopropyl-tridecyl-dimethyl-ammonium for 10 minutes or more under dirty conditions, and for 1 minute or more under clean conditions for both viruses (Poliovirus 1 and Adenovirus 5); using the 0.5% dilution a reduction of the virus titers higher than 4 Log10 was observed after incubation for 20 minutes or more under clean and dirty conditions (both viruses). A reduction of the HIV-1 titers higher that 4 Log10 was obtained using 2.5% isopropyl-tridecyl-dimethyl-ammonjum for 10 minutes or more under dirty conditions, and 1 minute or more under clean conditions. 0.5% dilution reduced the titers after incubation for 10 minutes or more under clean and dirty conditions. The mycobactericidial activity of the disinfectant formulation at 20°C for 60 minutes, 30 minutes, 10 minutes and 5 minutes was demonstrated. The bactericidal and fungicidal activities of Isopropyl-tridecyl-dimethyl-ammonium were also evaluated using a quantitative non-porous surface test at various concentrations. Conclusion: The data document the dynamics of the antimicrobial activity of the disinfectant tinder investigation, under in vitro controlled conditions and highlight the relatively low influence of organic material on its activity.
- Subjects
NIVELLES (Belgium); BELGIUM; ANTI-infective agents; DISINFECTION &; disinfectants; SURGICAL instruments; STAPHYLOCOCCUS aureus; ESCHERICHIA coli; ORGANIC compounds; QUANTITATIVE research
- Publication
Anaesthesia, Pain & Intensive Care, 2008, Vol 12, Issue 2, p54
- ISSN
1607-8322
- Publication type
Article