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- Title
Effect of Heracleum persicumm oil and alcoholic extracts on sperm parameters and chromatin quality in mice.
- Authors
Taghizabet N.; Mangoli E.; Anbari F.; Talebi A. R.; Masoodi A.
- Abstract
Introduction: Seminal plasma from infertile men has lower antioxidant levels than that of fertile men, particularly of patients whose semen have poor sperm motility. Evaluation of the importance and the effects of plant derived drugs on fertility of laboratory animals have long been recognized. Antioxidant activity has been reported from Heracleum persicum (Golpar). Materials and Methods: 80 adult male mice (10 weeks old, 35 gr) were divided to 3 groups: group 1 received hydroalcoholic extract (1000 mg/kg, ip), group 2 received oil extract (200 ml/kg, ip) and group 3 serving as the sham control group that received water. After 35 days, the cauda epididymis of each mouse was dissected and placed in 1 mL of pre-warm Ham's F10 culture medium for 30 min. The swim-out spermatozoa were analyzed for count, motility, morphology and viability. The sperm chromatin quality and DNA integrity, was evaluated with Aniline blue (AB), Toluidine blue (TB), Acridine orange (AO) and Chromomycin A3 (CMA3) staining. Results: In sperm analysis, progressive and nonprogressive motility were significantly differences between groups. Regard to sperm chromatin quality, the results of TB and AO tests showed statically significant differences between groups, but in AB and CM A3 staining, we didn't see any differences between them. So we can say that the Heracleum persicum extracts doesn't have any detrimental effects on histoneprotamines replacement during the testicular phase of sperm chromatin packaging. Conclusion: According to our results, Heracleum persicum extracts as an antioxidant although improved sperm motility but may influence the male fertility potential via affecting DNA/ chromatin quality in mice.
- Subjects
EXTRACTS; CHROMATIN; SPERM motility
- Publication
Iranian Journal of Reproductive Medicine, 2015, p80
- ISSN
1680-6433
- Publication type
Abstract