We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Evaluation of Spermicidal Activity of Mi-Saponin A.
- Authors
Saha, P.; Majumdar, S.; Pal, D.; Pal, B. C.; Kabir, S. N.
- Abstract
The seed extracts of Madhuca latifolia were reported to have spermicidal activity. The current investigation identified the spermicidal component of the extracts and evaluated its spermicidal potential in vitro. As characterized by infrared, mass, and nuclear magnetic resonance (NMR) spectral analyses, Mi-saponin A (MSA) was found to be the most potent component among a mixture of saponins. The mean effective concentrations of MSA that induced irreversible immobilization were 320 µg/mL for rat and 500 µg/mL for human sperm, as against the respective concentrations of 350 and 550 µg/mL of nonoxynol 9 (N-9). The mode of spermicidal action was evaluated by a battery of tests including (a) double fluoroprobe staining for spermviability, (b) hypoosmotic swelling test and, assays for 5′ nucleotidase and acrosin for physiological integrity of sperm plasma membrane, (c) scanning and transmission electron microscopy for sperm membrane ultrastructure, and (d) plasma membrane lipid peroxidation (LPO). The observations, taken together,were interpreted to mean that the spermicidal effect of MSA involved increased membrane LPO leading to structural and functional disintegration of sperm plasma membrane and acrosomal vesicle. A comparative in vitro cytotoxicity study in human vaginal keratocyte (Vk2/E6E7) and endocervical (End/E6E7) cell lines demonstrated that the 50% cell cytotoxicity (CC50) values, and consequently the safety indices, for MSA were ≥ 8-fold higher as compared to those of N-9. In conclusion, MSA is a potent spermicidal molecule that may be explored further for its suitability as an effective component of vaginal contraceptive.
- Subjects
SAPONINS; SPERMICIDES; PEROXIDATION; VAGINAL contraceptives; MADHUCA latifolia; NUCLEAR magnetic resonance
- Publication
Reproductive Sciences, 2010, Vol 17, Issue 5, p454
- ISSN
1933-7191
- Publication type
Article
- DOI
10.1177/1933719110361378