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- Title
Induction of Osmotolerance by Staphylococcus sciuri HP3 in Lens esculenta Var. Masoor 93 under NaCl Stress.
- Authors
Qurashi, Aisha Waheed; Sabri, Anjum Nasim
- Abstract
Agricultural output is greatly reduced in saline soil. Bacterial adaptation to salt stress in saline soil can be helpful for reducing the detrimental effects of salts in saline soil. This study was conducted to assess the impact of salt stress on efficiency of isolate HP3 Staphylococcus (Staph.) sciuri (previously isolated from histoplane of maize plant) in counteracting the adverse effect of salinity. The response of bacterial growth in the presence or absence of osmolytes (proline, glycine betaine and choline) at varying salt stress (0, 0.5, 1, 1.5, 2 and 2.5 molar NaCl) in LB-broth and M9 medium, bacterial osmolyte accumulation, biofilm formation and plant growth promoting potential was determined. Plant growth was measured in terms of seedling length, total soluble proteins and sugars contents of inoculated Lens esculenta Var. Masoor 93 seedlings in laboratory conditions under salt stress. The results showed that bacterial growth decreased with higher salinity (1.5-2.5 M NaCl). The biofilm formation of strain was visible as gummy colonies (0.5 M NaCl). Crystal violet ring assay showed a tendency of HP3 for biofilm formation at 0.5 to 2.0 M NaCl stress but no biofilm ring was observed at 0 or 2.5 M NaCl stress. However, highest on glass slides at 2.0 M NaCl stress. In the microtitre plate assay, biofilm was generally high at 1-1.5 M NaCl stress. However, exogenous osmolytes (especially proline) favoured bacterial growth and biofilm at high salt stress with little exceptions. In plant growth experiments, there was a significant reduction (P<0.05) in seedling length (56%) at high NaCl stress. Bacterial inoculations significantly improved the seedling length, Protein (31%) and total soluble sugars (81%) of seedlings. Accumulation of osmolytes and biofilm formation at salinity actually shows the promising strategies of stress tolerance that helped the Staph. sciuri to survive and improving plant growth under salt stress.
- Subjects
STAPHYLOCOCCUS; PLANT growth; LENTILS; EFFECT of stress on plants; EFFECT of salt on plants; SOIL salinity
- Publication
Pakistan Journal of Life & Social Sciences, 2016, Vol 14, Issue 1, p42
- ISSN
1727-4915
- Publication type
Article