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- Title
Hsa_circ_0000520 Promotes Non-Small Cell Lung Cancer Progression through the miR-1258/AKT3 Axis.
- Authors
Han, Lan; Yang, Hanxue; Wei, Wei; Hu, Fen; Yuan, Limei
- Abstract
Background. There are several previous studies suggesting that circular RNAs (circRNAs) are involved in tumorigenesis of non-small cell lung cancer (NSCLC). Nevertheless, the role of circRNA_0000520 (circ_0000520) in this disease has not yet been studied. Methods. circ_0000520, microRNA (miR)-1258, and AKT serine/threonine kinase 3 (AKT3) mRNA expression levels were detected by qPCR. CCK-8, EdU, and Transwell assays were utilized to detect NSCLC cells' malignant biological behaviors. The targeted relationship between miR-1258 and AKT3 3′-UTR or circ_0000520 was verified through the dual-luciferase reporter gene assay. Western blotting was utilized to measure the AKT3 expression after circ_0000520 and miR-1258 were selectively regulated. Results. circ_0000520 was upregulated in NSCLC. Highly expressed circ_0000520 is linked to the NSCLC patient's advanced TNM stage and lymph node metastasis. circ_0000520 overexpression facilitated NSCLC cell growth, migration, and invasion. miR-1258 was identified as the downstream target of circ_0000520. miR-1258 overexpression weakened the effect of circ_0000520 overexpression on NSCLC cells. miR-1258 targeted and inhibited AKT3. circ_0000520 positively regulated the AKT3 expression in NSCLC cells by sponging miR-1258. Conclusion. circ_0000520 upregulates AKT3 by competitively binding with miR-1258 to facilitate NSCLC progression.
- Subjects
LUNG cancer risk factors; CIRCULAR RNA; DISEASE progression; ANIMAL experimentation; WESTERN immunoblotting; MICRORNA; GENE expression; TRANSFERASES; OXIDOREDUCTASES
- Publication
Journal of Oncology, 2022, Vol 2022, p1
- ISSN
1687-8450
- Publication type
Article
- DOI
10.1155/2022/3676685