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- Title
Cloning and functioning analysis of transcription factor GmbHLH response to dehydration stress.
- Authors
CHEN Li-miao; SHA Ai-hua; ZHANG Chan-juan; SHAN Zhi-hui; ZHANG Xiao-juan; CHEN Shui-lian; ZHU Xiao-ling; LIU Bao-hong; QI Lin; ZHOU Xin-an; LI Wen-bin
- Abstract
Drought stress seriously affects plant growth and development as well as reduces quality and productivity of crops. In order to gain some key functional genes in response to dehydration/drought stress, a digital gene Expression Tag profile of two drought tolerance and sensitivity materials under dehydration had been constructed in our previous study. In the research, a gene encoding bHLH transcription factor, namely GmbHLH25, was identified from expression profile. This gene was significantly induced in leaves and roots under dehydration and the expression showed an obvious difference between two materials. Expression level of GmbHLH25 gene was validated by real - time quantitative PCR. The CDS sequence of GmbHLH had been cloned from two materials. GmbHLH25 gene encodes 368 aa and contains one basic Helix - Loop - Helix (bHLH) motif comprised of 50 amino acid. The homology tree demonstrated that GmbHLH25 was at the same evolutionary branch with homologous proteins of Lotus corniculatus and Medicago. Subcellular localization revealed that GmbHLH25 was distributed preferentially to nucleus. PCR and RT - PCR results showed that GmbHLH25 gene had been integrated into tobacco genome. Overexpression of GmbHLH25 gene improved drought and salt tolerance capacities under water - deficit and high salt conditions. This study will help to clarify the moleculear mechanisms of plants response to drought.
- Subjects
PLANT clones; DEHYDRATION; AGRICULTURAL productivity; GENE expression in plants; GENETIC transcription; POLYMERASE chain reaction; PLANTS
- Publication
Chinese Journal of Oil Crop Sciences, 2013, Vol 35, Issue 6, p630
- ISSN
1007-9084
- Publication type
Article
- DOI
10.7505/j.issn.1007-9084.2013.06.003