We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
The Rift Valley Fever virus protein NSm and putative cellular protein interactions.
- Authors
Engdahl, Cecilia; N„slund, Jonas; Lindgren, Lena; Ahlm, Clas; Bucht1,2*, G”ran
- Abstract
Rift Valley Fever is an infectious viral disease and an emerging problem in many countries of Africa and on the Arabian Peninsula. The causative virus is predominantly transmitted by mosquitoes and high mortality and abortion rates characterize outbreaks in animals while symptoms ranging from mild to life-threatening encephalitis and hemorrhagic fever are noticed among infected humans. For a better prevention and treatment of the infection, an increased knowledge of the infectious process of the virus is required. The focus of this work was to identify protein-protein interactions between the non-structural protein (NSm), encoded by the M-segment of the virus, and host cell proteins. This study was initiated by screening approximately 26 million cDNA clones of a mouse embryonic cDNA library for interactions with the NSm protein using a yeast two-hybrid system. We have identified nine murine proteins that interact with NSm protein of Rift Valley Fever virus, and the putative protein-protein interactions were confirmed by growth selection procedures and β-gal activity measurements. Our results suggest that the cleavage and polyadenylation specificity factor subunit 2 (Cpsf2), the peptidyl-prolyl cistrans isomerase (cyclophilin)-like 2 protein (Ppil2), and the synaptosome-associated protein of 25 kDa (SNAP-25) are the most promising targets for the NSm protein of the virus during an infection.
- Subjects
RIFT Valley fever; ARBOVIRUS diseases; VIRAL hepatitis; PROTEIN-protein interactions; PUBLIC health research
- Publication
Virology Journal, 2012, Vol 9, Issue 1, p139
- ISSN
1743-422X
- Publication type
Article
- DOI
10.1186/1743-422X-9-139