We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Avaliação do desempenho de teste rápido e de dois imunoensaios automatizados para Sars-CoV-2.
- Authors
Castejon, Márcia J.; Yamashiro, Rosemeire; Oliveira, Elaine L.; Silveira, Edilene R. P.; Hong, Marisa A.; Oliveira, Carmem Aparecida F.; Silva, Valéria O.; Ahagon, Cintia M.; Lima, Ana Késia S.; Lindoso, José Angelo L.; Brígido, Luís Fernando M.
- Abstract
Introduction: Due to urgency and demand of a response to the Covid-19 pandemic, numerous Sars-CoV-2 immunoassays have been rapidly developed. Objective: This study aimed at assessing the performance of rapid Sars-CoV-2 antibody test in comparison to high-throughput serological assays. Methods: A total of 86 serum samples were evaluated in the three assays: a lateral flow immunoassay – Wondfo Sars-CoV-2 Antibody Test (WRT) – and two chemiluminescence immunoassays: Elecsys Anti-Sars-CoV-2 (ECLIA), and Sars-CoV-2 IgG (CMIA-IgG). Results: The estimated diagnostic sensitivities of serological tests in the evaluation of serum samples from the epidemiological survey were: WRT 59.0% [95% confidence interval (CI) 43.4%-72.9%], ECLIA 66.7% (51%-79.4%), and CMIA-IgG 61.5% (47.1%-73%). Meanwhile, the estimated diagnostic specificity was for WRT 78.7% (95% CI 65.1%-88%), ECLIA 72.3% (58.2%-83.1%), and CMIA-IgG 76.6% (74%-95.5%). The sensitivity and specificity values were lower than manufacturers’ claimed. Although 16.2% (14/86) of serological results were discordant among the three Sars-CoV-2 serological assays, the degree of agreement by the kappa index was adequate: WRT/CMIA-IgG [0.757 (95% CI 0.615-0.899)], WRT/ECLIA [0.715 (0.565-0.864)], and ECLIA/CMIA-IgG [0.858 (0.748-0.968)]. Conclusion: The serological testing may be a useful diagnostic tool, which reinforces its careful evaluation, and, as well as the correct time to use it.
- Subjects
COVID-19 testing; IMMUNOASSAY; EPIDEMIOLOGY; IMMUNOGLOBULIN G; MEDICAL statistics
- Publication
Jornal Brasileiro de Patologia e Medicina Laboratorial, 2021, Vol 57, p1
- ISSN
1676-2444
- Publication type
Article
- DOI
10.5935/1676-2444.20210040