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- Title
Two-step cleavage of hairpin RNA with 5' overhangs by human DICER.
- Authors
Ando, Yoshinari; Maida, Yoshiko; Morinaga, Ayako; Burroughs, Alexander M; Kimura, Ryuichiro; Chiba, Joe; Suzuki, Harukazu; Masutomi, Kenkichi; Hayashizaki, Yoshihide
- Abstract
Background: DICER is an RNase III family endoribonuclease that processes precursor microRNAs (pre-miRNAs) and long double-stranded RNAs, generating microRNA (miRNA) duplexes and short interfering RNA duplexes with 20~23 nucleotides (nts) in length. The typical form of pre-miRNA processed by the Drosha protein is a hairpin RNA with 2-nt 3' overhangs. On the other hand, production of mature miRNA from an endogenous hairpin RNA with 5' overhangs has also been reported, although the mechanism for this process is unknown. Results: In this study, we show that human recombinant DICER protein (rDICER) processes a hairpin RNA with 5' overhangs in vitro and generates an intermediate duplex with a 29 nt-5' strand and a 23 nt-3' strand, which was eventually cleaved into a canonical miRNA duplex via a two-step cleavage. The previously identified endogenous pre-miRNA with 5' overhangs, pre-mmu-mir-1982 RNA, is also determined to be a substrate of rDICER through the same two-step cleavage. Conclusions: The two-step cleavage of a hairpin RNA with 5' overhangs shows that DICER releases double-stranded RNAs after the first cleavage and binds them again in the inverse direction for a second cleavage. These findings have implications for how DICER may be able to interact with or process differing precursor structures.
- Subjects
RNA; HAIRPIN (Genetics); RECOMBINANT proteins; NUCLEOTIDES; MICRORNA
- Publication
BMC Molecular Biology, 2011, Vol 12, Issue 1, p1
- ISSN
1471-2199
- Publication type
Article
- DOI
10.1186/1471-2199-12-6