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- Title
Development of specific PCR primer sets for detecting Pantoea dispersa, a potential biocontrol agent against rice seed-borne diseases caused by Burkholderia pathogens.
- Authors
Kouzai, Yusuke; Akimoto-Tomiyama, Chiharu
- Abstract
Pantoea dispersa is a plant-associated bacterium that often has beneficial effects on plants. In this study, we demonstrated that P. dispersa BB1, a previously identified potential biocontrol agent against the seedling rot pathogen Burkholderia glumae, could effectively suppress the symptoms of seedling blight caused by Burkholderia plantarii. To facilitate the isolation and utilization of P. dispersa as a biocontrol agent, a comparative genome analysis was performed to develop novel PCR primer sets for specifically detecting P. dispersa genes. Among the developed primer sets, the Pd725 set could detect 0.3 pg of genomic DNA of P. dispersa and evaluate the colonization of P. dispersa in plants. Using this primer set, we demonstrated that the colonization of P. dispersa in rice seeds infected with B. glumae and B. plantarii was significantly higher than that in non-infected seeds. This suggested that infection by Burkholderia pathogens promoted the colonization of P. dispersa. The Pd725 primer set could serve as a valuable tool for monitoring the colonization of P. dispersa in crops and evaluating potential new biocontrol strains.
- Subjects
RICE diseases &; pests; BURKHOLDERIA; BURKHOLDERIA infections; BIOLOGICAL pest control agents; PATHOGENIC microorganisms; RICE seeds; BACTERIAL diseases
- Publication
Journal of General Plant Pathology, 2024, Vol 90, Issue 1, p42
- ISSN
1345-2630
- Publication type
Article
- DOI
10.1007/s10327-023-01154-0