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- Title
Comparison of separation modes for microchip electrophoresis of proteins.
- Authors
Samarasinghe, Thushara N.; Zeng, Yong; Johnson, Carey K.
- Abstract
Separation of a set of model proteins was tested on a microchip electrophoresis analytical platform capable of sample injection by two different electrokinetic mechanisms. A range of separation modes—microchip capillary zone electrophoresis, microchip micellar electrokinetic chromatography, and nanoparticle‐based sieving—was tested on glass and polydimethylsiloxane/glass microchips and with silica‐nanoparticle colloidal arrays. The model proteins calmodulin (18 kiloDalton), bovine serum albumin (66 kDa), and concanavalin (106 kDa) were labeled with Alexa Fluor 647 for laser‐induced fluorescence detection. The best separation and resolution were obtained in a silica‐nanoparticle colloidal array chip.
- Subjects
MICROCHIP electrophoresis; CAPILLARY electrophoresis; MICELLAR electrokinetic chromatography; LASER-induced fluorescence; PROTEIN models; SERUM albumin
- Publication
Journal of Separation Science, 2021, Vol 44, Issue 3, p744
- ISSN
1615-9306
- Publication type
Article
- DOI
10.1002/jssc.202000883