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- Title
Polyaniline Based Electrochemical Biosensor for α‐Fetoprotein Detection Using Bio‐functionalized Nanoparticles as Detection Probe.
- Authors
Amarnath, Chellachamy Anbalagan; Sawant, Shilpa N.
- Abstract
Composite of polyaniline, bovine serum albumin and silver nanoparticles was used as the enriched immobilization matrix (EIM)‐cum‐electrode material for detection of liver cancer biomarker, α‐fetoprotein (AFP) in blood serum. In the sandwich biosensor, signal amplification was bestowed by EIM and detection probe. EIM entrapped large amount of capture antibody due to its higher surface area and amine‐rich nature, whereas detection probe enhanced the electrochemical signal during its binding with AFP. Biosensor exhibited excellent sensitivity, selectivity, reproducibility with a detection limit of 4.7 pg/mL. The amplification strategy adopted herein has potential for clinical detection of cancer biomarkers in point‐of‐care diagnostics.
- Subjects
BIOSENSORS; POLYANILINES; DNA probes; ALPHA fetoproteins; SERUM; SILVER nanoparticles; SERUM albumin; LIVER cancer
- Publication
Electroanalysis, 2020, Vol 32, Issue 11, p2415
- ISSN
1040-0397
- Publication type
Article
- DOI
10.1002/elan.202060219