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- Title
Regulation of the Caenorhabditis elegans posterior hox gene egl- 5 by microRNA and the polycomb-like gene sop- 2.
- Authors
Zhang, Hongjie; Emmons, Scott W.
- Abstract
In Caenorhabditis elegans, the domains of Hox gene expression are controlled by the novel global regulatory gene sop- 2. We identified a region located 3′ of the Hox gene egl- 5 that promotes ectopic expression of an egl- 5 reporter gene in a sop- 2 mutant. SOP-2 could directly block positive regulatory factors acting in this region, or it could block their expression. We identified three possible miRNA binding sites within the egl- 5 3′ untranslated region (UTR). Cognate microRNAs are expressed in relevant tissues and can block egl- 5 expression when expressed from a transgene. Mutation of the putative binding sites in the egl- 5 3′UTR resulted in a modest degree of misexpression of a minimal egl- 5 reporter gene, suggesting that microRNAs may contribute to the tight restriction of egl- 5 expression to particular cell lineages. Developmental Dynamics 238:595-603, 2009. © 2009 Wiley-Liss, Inc.
- Publication
Developmental Dynamics, 2009, Vol 238, Issue 3, p595
- ISSN
1058-8388
- Publication type
Article
- DOI
10.1002/dvdy.21876