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- Title
Influence of hormones and hormone metabolites on the growth of schwann cells derived from embryonic stem cells and on tumor cell lines expressing variable levels of neurofibromin.
- Authors
Roth, Therese M.; Ramamurthy, Poornapriya; Muir, David; Wallace, Margaret R.; Zhu, Yuan; Chang, Lou; Barald, Kate F.
- Abstract
Loss of neurofibromin, the protein product of the tumor suppressor gene neurofibromatosis type 1 (NF1), is associated with neurofibromas, composed largely of Schwann cells. The number and size of neurofibromas in NF1 patients have been shown to increase during pregnancy. A mouse embryonic stem cell (mESC) model was used, in which mESCs with varying levels of neurofibromin were differentiated into Schwann-like cells. NF1 cell lines derived from a malignant and a benign human tumor were used to study proliferation in response to hormones. Estrogen and androgen receptors were not expressed or expressed at very low levels in the NF1+/+ cells, at low levels in NF1+/−cells, and robust levels in NF1−/−cells. A 17β-estradiol (E2) metabolite, 2-methoxy estradiol (2ME2) is cytotoxic to the NF1−/− malignant tumor cell line, and inhibits proliferation in the other cell lines. 2ME2 or its derivatives could provide new treatment avenues for NF1 hormone-sensitive tumors at times of greatet hormonal influence. Developmental Dynamics 237:513-524, 2008. © 2008 Wiley-Liss, Inc.
- Publication
Developmental Dynamics, 2008, Vol 237, Issue 2, p513
- ISSN
1058-8388
- Publication type
Article
- DOI
10.1002/dvdy.21430