We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Effect of BMP-12, TGF-β1 and autologous conditioned serum on growth factor expression in Achilles tendon healing.
- Authors
Heisterbach PE; Todorov A; Flückiger R; Evans CH; Majewski M; Heisterbach, Patricia E; Todorov, Atanas; Flückiger, Rudolf; Evans, Christopher H; Majewski, Martin
- Abstract
<bold>Purpose: </bold>Achilles tendon ruptures are devastating and recover slowly and incompletely. There is a great demand for biomolecular therapies to improve recovery, yet little is understood about growth factors in a healing tendon. Here, the role of growth factors during tendon healing in a rat model and their reaction to single and multiple growth factor treatment are explored.<bold>Methods: </bold>Rat tendons were transected surgically and resutured. The expression of bFGF, BMP-12, VEGF and TGF-β1 was assessed by immunohistochemical analysis one to 8 weeks after surgery. Paracrine effects of TGF-β1 or BMP-12 added by adenoviral transfer, as well as the effect of autologous conditioned serum (ACS) on growth factor expression, were evaluated.<bold>Results: </bold>bFGF, BMP-12 and VEGF expression was highest 1 week after transection. bFGF and BMP-12 declined during the remaining period whereas VEGF expression persisted. TGF-β1 expression dramatically increased after 8 weeks. ACS treatment increased bFGF (P = 0.007) and BMP-12 (P = 0.004) expression significantly after 8 weeks. Also overall expression of bFGF, BMP-12 and TGF-β1 regardless of time point was significantly greater than controls with ACS treatment (P < 0.05). Both BMP-12 and TGF-β1 treatments had no significant effect. No effect was observed in VEGF with any treatment.<bold>Conclusion: </bold>bFGF, BMP-12, VEGF and TGF-β1 are differentially expressed during tendon healing. Additional BMP-12 or TGF-β1 has no significant influence, whereas ACS generally increases expression of all factors except VEGF. Staged application of multiple growth factors may be the most promising biomolecular treatment.
- Publication
Knee Surgery, Sports Traumatology, Arthroscopy, 2012, Vol 20, Issue 10, p1903
- ISSN
0942-2056
- Publication type
journal article