We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
FcγRII Activation Induces Cell Surface Ceramide Production which Participates in the Assembly of the Receptor Signaling Complex.
- Authors
Korzeniowski, Marek; Abdel Shakor, Abo Bakr; Makowska, Agnieszka; Drzewiecka, Anna; Bielawska, Alicja; Kwiatkowska, Katarzyna; Sobota, Andrzej
- Abstract
We studied an involvement of various cellular ceramide pools in signaling of immunoreceptor FcγII (FcγRII). The cell surface ceramide level was assessed by a technique based on binding of ceramide probes to intact cells. Total cellular ceramide was estimated by radioactive measurements. The activity of sphingomyelinases was measured by NBD-ceramide release while immunoprecipitation and immunoblotting were applied to analyze protein tyrosine phosphorylation. A complex pattern of protein phosphorylation was found to accompany FcγRII activation and the phosphorylation was either diminished by imipramine or increased by B13, modulators of acid sphingomyelinase and acid ceramidase activity. The effects of the drugs on the phosphorylation of FcγRII and NTAL were prominent and correlated with a reduction of the cell surface ceramide production by imipramine and an augmentation of the ceramide generation by B13. The ceramide generation followed activation of acid sphingomyelinase and preceded that of neutral sphingomyelinase. The level of cell surface ceramide was additionally elevated by exogenous bacterial sphingomyelinase, but only at later stages of the receptor activation. The total mass of ceramide was diminished in the course of receptor activation pointing to an engagement of enzymes metabolizing ceramide. The data indicate that FcγRII activates enzymes of the sphingomyelin cycle which affect various sphingomyelin/ceramide pools in a cell. Copyright © 2007 S. Karger AG, Basel
- Subjects
PHOSPHORYLATION; CELL membranes; IMMUNOBLOTTING; AMINO acids; CHEMICAL reactions
- Publication
Cellular Physiology & Biochemistry (Karger AG), 2007, Vol 20, Issue 5, p347
- ISSN
1015-8987
- Publication type
Article
- DOI
10.1159/000107520