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- Title
Structure and biosynthesis of deoxy-polyamine in Xenorhabdus bovienii.
- Authors
Wenski, Sebastian L.; Berghaus, Natalie; Keller, Nadine; Bode, Helge B.
- Abstract
Polyamine moieties have been described as part of the fabclavine and zeamine family of natural products. While the corresponding biosynthetic gene clusters have been found in many different proteobacteria, a unique BGC was identified in the entomopathogenic bacterium Xenorhabdus bovienii. Mass spectrometric analysis of a X. bovienii mutant strain revealed a new deoxypolyamine. The corresponding biosynthesis includes two additional reductive steps, initiated by an additional dehydratase (DH) domain, which was not found in any other Xenorhabdus strain. Moreover, this DH domain could be successfully integrated into homologous biosynthesis pathways, leading to the formation of other deoxy-polyamines. Additional heterologous production experiments revealed that the DH domain could act in cis as well as in trans.
- Subjects
XENORHABDUS; GENE clusters; BIOSYNTHESIS; NATURAL products; ENTOMOPATHOGENIC fungi; UNSATURATED fatty acids; PROTEOBACTERIA
- Publication
Journal of Industrial Microbiology & Biotechnology, 2021, Vol 48, Issue 3/4, p1
- ISSN
1367-5435
- Publication type
Article
- DOI
10.1093/jimb/kuab006