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- Title
Evaluating Bioplastic Production of Glucose Metabolic Perturbations Pseudomonas Putida Strains from Waste Frying Oil.
- Authors
Thi-Ngoc-Yen Nguyen; Nguyen-Nguyen-Nhat Vo; Son-Tung Nguyen; Tu-Anh Nguyen; Huynh-Nhu Mai; Minh-Thai Nguyen
- Abstract
Waste frying oil (WFO) can take a heavy toll on people' health and the environment; however, they can still be useful in reality if they are appropriately collected and recycled. Specifically, we can take their advantages to create a nutritious carbon source for bacteria growth. In this research, due to a strong desire to enhance the value of such products, Pseudomonas putida KT2440 is employed to produce polyhydroxyalkanoates (PHA) which is a group of natural biodegradable polyesters synthesized by microorganisms. Two deficient glucose assimilation chassis strains, which are the glk and gcd created through knock-out genes encoding glucose dehydrogenase (gcd) and glucoskinase (glk) respectively, are compared with P.putida KT2440 WildType (WT) for optimization of PHA production. Batch cultivation coupled with an approach of standard submerged fermentation is used in order to monitor the biosynthesis of PHA and the growth profile of producer strains. The results indicate a significant improvement of PHA yields when using WFO at a concentration of 20 g/L and applying gene knock-outs, especiallyglk. Although there are still drawbacks, our results basically proclaim that the amount of gained PHA by using WFO is higher than glycerol which is the material of choice for PHA production. Additionally, the successful incorporation of PHA with Tylosin, an antibiotic belonging to the macrolide group, could be the fresh direction in fights against antibiotics resistance. To sum up, P. putida can convert greasy wastes into biopolymers boasting wide applications in diverse fields, which paves the way to develop the straightforward procedure to recycle and value this waste on a real life.
- Subjects
BIODEGRADABLE plastics; PSEUDOMONAS putida; FATS &; oils; FRYING; GLUCOSE metabolism; POLYHYDROXYALKANOATES
- Publication
CET Journal - Chemical Engineering Transactions, 2023, Vol 101, p241
- ISSN
1974-9791
- Publication type
Article
- DOI
10.3303/CET23101041