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- Title
Effect of pain related of sex and estrous cycle on blood plasma glucose, free fatty acids and corticosterone in rats.
- Authors
de Moraes, Elzira Diniz; Zaia, Cássia Thaís Bussamara Vieira; Zaia, Dimas Augusto Morozin; dos Santos, Angelo Alexander Torres; Ribeiro, Rachel Cezar de Andrade; da Silva, Andreia Lopes
- Abstract
Sex differences related with pain have been studied and evidences suggesting influence of sex steroid hormones on the thresholds of pain. Experimental nociception has been test using formalin as a model of nociceptive stimulus. Association of stress, pain and metabolic and hormonal changes has not been explored. The aim of this study was to compare metabolic and hormonal changes between male rats and female rats in proestrus and estrus cycle after painful stimulus by formalin into the masseter muscle. Male and female Wistar rats (200-250 g b.w.) were submitted to an injection of formalin (F, 1.5%) or saline (S, 9.9%) into the masseter muscle and after 0 (N, control group without injection), 5, 15, 30 or 60 minutes they were decapitate and blood was collected to measure biochemical parameters. Plasma estradiol concentration (pg dL-1) was significantly higher in proestrus (106.3 ± 4.3, n = 45, p <0.05) group compared to the estrous group (89.4 ± 3.5, n = 43). Blood plasma concentration of glucose (mg dL-1) was increased after 5 and 15 minutes of injection of formalin or saline in the animals, but in the estrus group the increase was bigger than in the others. Free fatty acids levels increased in the estrous group after 5, 15 and 30 minutes and also the corticosterone levels and these concentrations were significantly different (p <0.05) from either male or female animals in proestrus state. The results obtained suggesting that estradiol is related to a sensibility to pain and the estrus stage is related to stress and the estrous cycle has a modulator effect on pain and nociceptive sensibility.
- Subjects
SEXUAL dimorphism; ESTRUS; BLOOD plasma; FATTY acids; CORTICOSTERONE; FORMALDEHYDE; ANIMAL models in research; RATS
- Publication
Acta Scientiarum: Biological Sciences, 2012, Vol 34, Issue 4, p451
- ISSN
1679-9283
- Publication type
Article
- DOI
10.4025/actascibiolsci.v34i4.10568