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- Title
Analysis of children with Chlamydophila (Chlamydia) pneumoniae and Mycoplasma pneumoniae respiratoryinfections by real-time PCR assay and serological tests.
- Authors
Otomo, Shinobu; Yamamura, Jun-Ichi; Hayashi, Erina; Nakamura, Tsuneyuki; Kakinuma, Hiroaki; Nakamoto, Yumi; Takahashi, Hiroaki; Karasawa, Tadahiro
- Abstract
We examined 73 children with respiratory infections for Chlamydophila (Chlamydia) pneumoniae and Mycoplasma pneumoniae using real-time PCR assay and serological tests. C. pneumoniae and M. pneumoniae infections were found in 11 (15.1%) and 6 (8.2%) cases, respectively. The sensitivities and specificities of real-time PCR versus definite diagnosis of acute infection were 63.6% and 100% for C. pneumoniae, and 100% and 100% for M. pneumoniae, respectively. C. pneumoniae PCR-negative results appeared to be due to poor growth of the organism. The sensitivity and specificity of ImmunoCard tests were 33.3% and 82.1%, respectively, indicating that the efficacy of rapid diagnosis was disputable. The present results suggest that real-time PCR is suitable for rapid diagnosis as a first screening test to determine first-line antibacterial agents to be used against these infectious diseases.
- Subjects
RESPIRATORY infections in children; CHLAMYDOPHILA infections; MYCOPLASMA pneumoniae infections; POLYMERASE chain reaction; DIAGNOSIS; MEDICAL screening; ANTI-infective agents
- Publication
APMIS, 2008, Vol 116, Issue 6, p477
- ISSN
0903-4641
- Publication type
Article
- DOI
10.1111/j.1600-0463.2008.00973.x