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- Title
Intracellular localization of a lipid transfer protein inVigna unguiculataseeds.
- Authors
de O. Carvalho, André; de S. Teodoro, Carlos Eduardo; Da Cunha, Maura; Okorokova-Façanha, Anna L.; Okorokov, Lev A.; Fernandes, K´tia V. S.; Gomes, Valdirene M.
- Abstract
Lipid transfer proteins (LTP) facilitate transfer of lipids between membranes in vitro. Up to now, they have been found to be localized basically in the plant cell wall and in compartments linked to lipid metabolism, such as glyoxysomes. Accordingly, LTP are considered to be involved in the plant defence against pathogen microbes and lipid metabolism. We herein show, by immunoelectron microscopy, that besides the cell wall, LTP are localized in the lumen of organelles which we suggest to be the protein storage vacuoles, as well as in vesicles similar to the lipid-containing ones and in the extracellular space ofVigna unguiculataseeds. To further characterize these organelles, we performed subcellular fractionation of membranes isolated from imbibed seeds on a sucrose-density gradient. The analysis of these fractions revealed that the lightest membrane vesicles, derived probably from PSV, contain LTP,α-TIP and K+ independent PPiase, but notγ-TIP and K+ stimulated PPiase. The presence of LTP and vicilins (typical storage protein) in the lumen of these vesicles was confirmed by immunoelectron microscopy. Taken together, the data suggest that the intracellular LTP in theV. unguiculataseeds are localized in protein storage vacuoles and in lipid-containing vesicles.
- Subjects
LIPIDS; PROTEINS; LIPID metabolism; COWPEA; PLANT vacuoles; SUCROSE
- Publication
Physiologia Plantarum, 2004, Vol 122, Issue 3, p328
- ISSN
0031-9317
- Publication type
Article
- DOI
10.1111/j.1399-3054.2004.00413.x