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- Title
Integrity of Escherichia coli P pili during biogenesis: properties and role of PapJ.
- Authors
Tennent, J. M.; Lindberg, F.; Normark, S.
- Abstract
The <em>papJ</em> gene of uropathogenic <em>Escherichia coli</em> is required to maintain the integrity of Galα(1 -4)Gal-binding P pili. Electron microscopy and ELISA have established that strains carrying the <em>papJ1</em> mutant allele have a large amount of pilus antigen free of the cells. In contrast to the whole pili released by strains unable to produce the PapH pilus anchor, the free <em>papJ1</em> pili consist of variably sized segments that appear to result from internal breakages to the pilus. The DNA sequence of <em>papJ</em> is presented and its gene product identified as an 18kD periplasmic protein that possesses homology with nucleotide-binding proteins. PapJ may function as a 'molecular chaperone' directly or indirectly establishing the correct assembly of PapA subunits in the P pilus.
- Subjects
GENES; PATHOGENIC microorganisms; ESCHERICHIA coli; ESCHERICHIA; ESCHERICHIA coli O157:H7; PILI (Microbiology); ELECTRON microscopy
- Publication
Molecular Microbiology, 1990, Vol 4, Issue 5, p747
- ISSN
0950-382X
- Publication type
Article
- DOI
10.1111/j.1365-2958.1990.tb00645.x