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- Title
Inhibitory effects of Lepidium sativum polysaccharide extracts on TNF-α production in Escherichia coli-stimulated mouse.
- Authors
Ahmad, Ajaz; Jan, Basit Latief; Raish, Mohammad; Alkharfy, Khalid M.; Ahad, Abdul; Khan, Altaf; Ganaie, Majid Ahmad; Hamidaddin, Mohammed Abbas Ali
- Abstract
The present study was designed to study the quantitative effects of extraction time, temperature and solvent to sample ratio on the yield of Lepidium sativum polysaccharides (LSP) using a Box-Behnken design. The activities of the optimized LSP extract were then tested in an in vivo experimental system of Escherichia coli (E. coli)-induced endotoxin shock. The optimal polysaccharide extraction conditions were established by the equation of regression and evaluation of the response surface contour plots: extraction time 5.2 h; temperature 95 °C and ratio of water to raw material 31.89 mL/g. Subsequently, an in vivo endotoxin shock was induced in mice with a single E. coli i.p. injection. Septic mice showed a substantial raise in the levels of tumor necrosis factor alpha (TNF-α) in plasma, whereas mice treated with LSP after E. coli injection showed considerable lower plasma levels of TNF-α (P < 0.05). These results suggest that LSP have beneficial effects when administered to mice with endotoxin shock by diminishing the pro-inflammatory response. The systemic activity of LSP indicated that the extract has a significant inhibitory effect against E. coli-induced inflammation by reducing the circulating levels of TNF-α. Further studies are warranted to explore the clinical implications of such observations.
- Subjects
LEPIDIUM; POLYSACCHARIDES; TUMOR necrosis factors; LABORATORY mice; PLANT extracts
- Publication
3 Biotech, 2018, Vol 8, Issue 6, p1
- ISSN
2190-572X
- Publication type
Article
- DOI
10.1007/s13205-018-1309-9