We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Development of a SFTSV DNA vaccine that confers complete protection against lethal infection in ferrets.
- Authors
Kwak, Jeong-Eun; Kim, Young-Il; Park, Su-Jin; Yu, Min-Ah; Kwon, Hyeok-Il; Eo, Sukyeong; Kim, Tae-Shin; Seok, Joon; Choi, Won-Suk; Jeong, Ju Hwan; Lee, Hyojin; Cho, Youngran; Kwon, Jin Ah; Jeong, Moonsup; Maslow, Joel N.; Kim, Yong-Eun; Jeon, Haili; Kim, Kee K.; Shin, Eui-Cheol; Song, Min-Suk
- Abstract
Although the incidence of severe fever with thrombocytopenia syndrome virus (SFTSV) infection has increased from its discovery with a mortality rate of 10–20%, no effective vaccines are currently available. Here we describe the development of a SFTSV DNA vaccine, its immunogenicity, and its protective efficacy. Vaccine candidates induce both a neutralizing antibody response and multifunctional SFTSV-specific T cell response in mice and ferrets. When the vaccine efficacy is investigated in aged-ferrets that recapitulate fatal clinical symptoms, vaccinated ferrets are completely protected from lethal SFTSV challenge without developing any clinical signs. A serum transfer study reveals that anti-envelope antibodies play an important role in protective immunity. Our results suggest that Gn/Gc may be the most effective antigens for inducing protective immunity and non-envelope-specific T cell responses also can contribute to protection against SFTSV infection. This study provides important insights into the development of an effective vaccine, as well as corresponding immune parameters, to control SFTSV infection. Severe fever with thrombocytopenia syndrome virus (SFTSV) is an emerging tick-borne virus with no specific treatment or vaccine available. Here, the authors develop a DNA vaccine for SFTSV that is protective against lethal challenge in ferrets and show that anti-envelope antibodies are important for protection.
- Subjects
DNA vaccines; FERRET; CD8 antigen; SYMPTOMS; VACCINE effectiveness; ANTIBODY formation; VIRAL envelope proteins
- Publication
Nature Communications, 2019, Vol 10, Issue 1, pN.PAG
- ISSN
2041-1723
- Publication type
Article
- DOI
10.1038/s41467-019-11815-4