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- Title
The Arabidopsis TRM61/TRM6 complex is a bona fide tRNA N<sup>1</sup>-methyladenosine methyltransferase.
- Authors
Tang, Jun; Jia, Pengfei; Xin, Peiyong; Chu, Jinfang; Shi, Dong-Qiao; Yang, Wei-Cai
- Abstract
tRNA molecules, which contain the most abundant post-transcriptional modifications, are crucial for proper gene expression and protein biosynthesis. Methylation at N1 of adenosine 58 (A58) is critical for maintaining the stability of initiator methionyl-tRNA (tRNAiMet) in bacterial, archaeal, and eukaryotic tRNAs. However, although research has been conducted in yeast and mammals, it remains unclear how A58 in plant tRNAs is modified and involved in development. In this study, we identify the nucleus-localized complex AtTRM61/AtTRM6 in Arabidopsis as tRNA m1A58 methyltransferase. Deficiency or a lack of either AtTRM61 or AtTRM6 leads to embryo arrest and seed abortion. The tRNA m1A level decreases in conditionally complemented Attrm61/LEC1pro::AtTRM61 plants and this is accompanied by reduced levels of tRNAiMet, indicating the importance of the tRNA m1A modification for tRNAiMet stability. Taken together, our results demonstrate that tRNA m1A58 modification is necessary for tRNAiMet stability and is required for embryo development in Arabidopsis.
- Subjects
TRANSFER RNA; ARABIDOPSIS; PROTEIN synthesis; PROTEIN expression; GENE expression
- Publication
Journal of Experimental Botany, 2020, Vol 71, Issue 10, p3024
- ISSN
0022-0957
- Publication type
Article
- DOI
10.1093/jxb/eraa100