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- Title
In Vivo Assessment of VCAM-1 Expression by SPECT/CT Imaging in Mice Models of Human Triple Negative Breast Cancer.
- Authors
Montemagno, Christopher; Dumas, Laurent; Cavaillès, Pierre; Ahmadi, Mitra; Bacot, Sandrine; Debiossat, Marlène; Soubies, Audrey; Djaïleb, Loic; Leenhardt, Julien; De Leiris, Nicolas; Dufies, Maeva; Pagès, Gilles; Hernot, Sophie; Devoogdt, Nick; Perret, Pascale; Riou, Laurent; Fagret, Daniel; Ghezzi, Catherine; Broisat, Alexis
- Abstract
Recent progress in breast cancer research has led to the identification of Vascular Cell Adhesion Molecule-1 (VCAM-1) as a key actor of metastatic colonization. VCAM-1 promotes lung-metastases and is associated with clinical early recurrence and poor outcome in triple negative breast cancer (TNBC). Our objective was to perform the in vivo imaging of VCAM-1 in mice models of TNBC. The Cancer Genomic Atlas (TCGA) database was analyzed to evaluate the prognostic role of VCAM-1 in TNBC. MDA-MB-231 (VCAM-1+) and control HCC70 (VCAM-1-) TNBC cells were subcutaneously xenografted in mice and VCAM-1 expression was assessed in vivo by single-photon emission computed tomography (SPECT) imaging using 99mTc-cAbVCAM1-5. Then, MDA-MB-231 cells were intravenously injected in mice and VCAM-1 expression in lung metastasis was assessed by SPECT imaging after 8 weeks. TCGA analysis showed that VCAM-1 is associated with a poor prognosis in TNBC patients. In subcutaneous tumor models, 99mTc-cAbVCAM1-5 uptake was 2-fold higher in MDA-MB-231 than in HCC70 (p < 0.01), and 4-fold higher than that of the irrelevant control (p < 0.01). Moreover, 99mTc-cAbVCAM1-5 uptake in MDA-MB-231 lung metastases was also higher than that of 99mTc-Ctl (p < 0.05). 99mTc-cAbVCAM1-5 is therefore a suitable tool to evaluate the role of VCAM-1 as a marker of tumor aggressiveness of TNBC.
- Subjects
BREAST cancer prognosis; ANIMAL experimentation; CELL adhesion molecules; CELL lines; COMPUTED tomography; GENE expression; HEALTH; INTRAVENOUS injections; LUNG tumors; METASTASIS; MICE; TECHNETIUM; TUMOR markers; XENOGRAFTS; BIBLIOGRAPHIC databases; SINGLE-photon emission computed tomography; IN vivo studies
- Publication
Cancers, 2019, Vol 11, Issue 7, p1039
- ISSN
2072-6694
- Publication type
Article
- DOI
10.3390/cancers11071039