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- Title
Expression of polycystin in mouse metanephros and extra- metanephric tissues.
- Authors
Griffin, Matthew D.; O'Sullivan, Deirdre A.; Torres, Vicente E .; Grande, Joseph P.; Kanwar, Yashpal S.; Kumar, Rajiv
- Abstract
Expression of polycystin in mouse metanephros and extra-metanephric tissues. The presence of messenger RNA for the mouse homologue of the polycystic kidney disease 1 gene (<em>PKDl</em>) was demonstrated by reverse transcription-polymerase chain reaction (RT-PCR) methods In mouse embryo messenger RNA. A single focus tor the <em>PKD1</em> gene was detected on mouse chromosome f7 by fluorescent <em>in situ</em> hybridization. Immunoprecipitation of proteins from [35S] methionine-labeled mouse metanephric explants with an anti-polycystin antibody (Pcl) revealed high molecular weight bands, the highest being >400 kDa. Immursoperosidase staining of mouse embryos with (Pcl) revealed expression of polycystin as early as day 8 gestation, the expression was seen in epithelial cells of the ureteric bud. in condensing blastemal cells of the developing metanepbros and subsequently, in cells of the nascent tubules. In addition. Pel immunoreactivity was seen in hepatocytes and biliary epithelium, cardiac and skeletal muscle, neural tissue, gut, and bronchial epithelium. In post-natal and adult mouse kidney and liver persistent slight to moderate immunoreactivity was observed. Immunofluorescent studies of cultured [3-day mouse metanephroi revealed polycystin expression in ureteric bud epithelium, early glomerular structures (that is, condensates. S-shaped and commashaped bodies) and in proximal and distal tubular epithelia. These data indicate that the mouse has a single gene homologous to human <em>PKDl</em> on chromosome 17, and polycystin is expressed in a variety of tissues during embryonic development Expression of polycystin in mouse metaaephros and extra-metanephric tissues. The presence of messenger RNA for the mouse homologue to the polycystic kidney disease I gene (<em>PKDl</em>) was demonstrated by reverse transcription-polymerase chain reaction (RT-PCR) methods In mouse embryo messenger RNA. A single focus tor the <em>PKD1</em> gene was detected on mouse chromosome 17 by fluorescent <em>in situ</em> hybridization. Immunoprecipitation of proteins from [35S] methionine-labeled mouse metanephric explants with an anti-poiycystin antibody (Pcl) revealed high molecular weight bands, the highest being >400 kDa. Immunoperoxidase staining of mouse embryos with Pcl revealed expression of polycystin as early as day 8 gestation, the expression was seen in epithelial cells of the ureteric bud. in condensing blastemal cells of the developing metanepbros and subsequently, in cells of the nascent tubules. In addition. Pcl immunoreactivity was seen in hepatocytes and biliary epithelium, cardiac and skeletal muscle, neural tissue, gut, and bronchial epithelium. In post-natal and adult mouse kidney and liver persistent slight to moderate immunoreactivity was observed. Immunofluorescent studies of cultured 13-day mouse metanephractivity revealed polycystin expression in ureteric bud epithelium, early glomerular structures (that is, condensates. S-shaped and comma-shaped bodies) and in proximal and distal tubular epithelia. These data indicate that the mouse has a single gene homologous to human <em>PKDl</em> on chromosome 17. and polycystin is expressed in a variety of tissues during embryonic development.
- Subjects
POLYMERASE chain reaction; MESSENGER RNA; RADIOLARIA; CHROMOSOME abnormalities; DNA polymerases; EPITHELIAL cells
- Publication
Kidney International, 1997, Vol 52, Issue 5, p1196
- ISSN
0085-2538
- Publication type
Article
- DOI
10.1038/ki.1997.444