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- Title
Decreased total and active urinary kallikrein in normotensive Dahl salt susceptible rats.
- Authors
Arbeit, Leonard A.; Serra, Susan R.
- Abstract
Abnormalities in the kallikrein-kinin system have been found in human and animal models of essential hypertension. The purpose of this study is to assess the kalikrein-kinin system in normotensive Dahl salt sensitive (S) and salt resistant (R) rats on a zero sodium diet. Urinary kalikrein was measured at 7 and 12 wk of age by different techniques. When kallikrein activity was assessed, by a kininogenase assay. S rats excreted 66% (p< 0.001) and 75% (P < 0.01) as much kallikrein as R rats at 7 and 12 wk of age. Using an artificial substrate method (Kabi S-2266). S rats excreted 30% (P < 0.001) and 56% (P < 0.05) as much kallikrein as R rats at 7 and 12 weeks, respectively. Using a technique to measure total kallikrein, S rats excreted 53% (P < 0.001) and 65% (P < 0.05) as much kallikrein as R rats at 7 and 12 wk of age. Normotensive S rats failed to increase maximally kallikrein activity or total kallikrein when the diet was switched from a .4% to 0.0064% sodium chloride diet. There was no difference in inhibitors, as measured by the recovery of purified kallikrein added to S and R urine (56 ± 21% vs. 53 ± 13%). Km values for S and R urinary kallikrein were similar (3.1 ± .5 × 10-5 vs. 2.6 ± .5 × 10-5 M/liter). Trypsin-activatable kallikrein was equivalent in the S and R rats on the .0064% and .4% sodium chloride diet. This study supports the hypothesis that there is diminished kallikrein activity in S rats, and that this is due to decreased kallikrein excretion rather than inhibitors in the urine, an abnormality in the enzyme, or an inactive enzyme.
- Subjects
KALLIKREIN; KININS; PANCREATIC secretions; ESSENTIAL hypertension; SODIUM; SALT
- Publication
Kidney International, 1985, Vol 28, Issue 3, p440
- ISSN
0085-2538
- Publication type
Article
- DOI
10.1038/ki.1985.150