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- Title
Novel method to analysis benzo[ a]pyrene in filter by liquid chromatography/tandem mass spectrometry: Application to assess mouth level benzo[ a]pyrene exposure.
- Authors
Zhang, Xiaotao; Hou, Hongwei; Shi, Longkai; Liu, Yong; Wang, An; Hu, Qingyuan
- Abstract
RATIONALE Benzo[a]pyrene (B[a]P) is a well-known carcinogenic compound produced from incomplete combustion of organic compounds. During cigarette smoking, cigarette filters trap a portion of mainstream smoke B[a]P and accurate measurement of B[a]P levels in cigarette filter can be used to estimate human exposure level of this compound. METHODS A rapid and sensitive method to quantify B[a]P levels trapped by cigarette filter was developed. The method is based on liquid chromatography/atmospheric pressure photoionization tandem mass spectrometry (LC/APPI-MS/MS). Validation was provided in smoked cigarette filter. RESULTS The method involved no extensive manual cleanup and concentration steps, hence is easy to use. The limit of detection was 0.064 ng/mL. The recovery ranged from 82.68% to 103.27% and the relative standard deviation (RSD) was less than 7%. A regression model between mainstream cigarette smoke and trapped B[a]P by cigarette filter was established (y = 2.5089x - 0.1851, R2 = 0.999, n = 6). This model was applied to estimate mouth-level B[a]P exposure of smokers. CONCLUSIONS The LC/APPI-MS/MS method developed in this work had an excellent throughput in estimating the mouth-level exposure under natural human smoking conditions. Mouth-level B[a]P exposure based on a study of three cigarettes (8, 10 and 13 mg tar levels) showed significant positive correlations with B[a]P trapped by cigarette filter measured (y = 2.5456x - 0.5056, R2 = 1, n = 249). Copyright © 2014 John Wiley & Sons, Ltd.
- Subjects
AROMATIC compounds spectra; PYRENE; LIQUID chromatography-mass spectrometry; CARCINOGENS; CIGARETTE filters; CIGARETTE smoke; REGRESSION analysis
- Publication
Rapid Communications in Mass Spectrometry: RCM, 2014, Vol 28, Issue 13, p1468
- ISSN
0951-4198
- Publication type
Article
- DOI
10.1002/rcm.6921