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- Title
Structure–Activity Relationship of Oleanane-Type Pentacyclic Triterpenoids on Nuclear Factor κB Activation and Intracellular Trafficking and N -Linked Glycosylation of Intercellular Adhesion Molecule-1.
- Authors
Nakano, Kaori; Yokota, Yuka; Vu, Quy Van; Lagravinese, Francesca; Kataoka, Takao
- Abstract
In our previous study, two oleanane-type pentacyclic triterpenoids (oleanolic acid and maslinic acid) were reported to affect the N-glycosylation and intracellular trafficking of intercellular adhesion molecule-1 (ICAM-1). The present study was aimed at investigating the structure–activity relationship of 13 oleanane-type natural triterpenoids with respect to the nuclear factor κB (NF-κB) signaling pathway and the expression, intracellular trafficking, and N-glycosylation of the ICAM-1 protein in human lung adenocarcinoma A549 cells. Hederagenin, echinocystic acid, erythrodiol, and maslinic acid, which all possess two hydroxyl groups, decreased the viability of A549 cells. Celastrol and pristimerin, both of which possess an α,β-unsaturated carbonyl group, decreased cell viability but more strongly inhibited the interleukin-1α-induced NF-κB signaling pathway. Oleanolic acid, moronic acid, and glycyrrhetinic acid interfered with N-glycosylation without affecting the cell surface expression of the ICAM-1 protein. In contrast, α-boswellic acid and maslinic acid interfered with the N-glycosylation of the ICAM-1 protein, which resulted in the accumulation of high-mannose-type N-glycans. Among the oleanane-type triterpenoids tested, α-boswellic acid and maslinic acid uniquely interfered with the intracellular trafficking and N-glycosylation of glycoproteins.
- Subjects
STRUCTURE-activity relationships; TRITERPENOIDS; GLYCOSYLATION; CARBONYL group; CELL adhesion; HYDROXYL group; INTERLEUKIN-1 receptors
- Publication
International Journal of Molecular Sciences, 2024, Vol 25, Issue 11, p6026
- ISSN
1661-6596
- Publication type
Article
- DOI
10.3390/ijms25116026