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- Title
Is the Neuropeptide PEN a Ligand of GPR83?
- Authors
Giesecke, Yvonne; Asimi, Vahid; Stulberg, Valentina; Kleinau, Gunnar; Scheerer, Patrick; Koksch, Beate; Grötzinger, Carsten
- Abstract
G protein-coupled receptor 83 (GPR83) is a class A G protein-coupled receptor with predominant expression in the cerebellum and proposed function in the regulation of food intake and in anxiety-like behavior. The neuropeptide PEN has been suggested as a specific GPR83 ligand. However, conflicting reports exist about whether PEN is indeed able to bind and activate GPR83. This study was initiated to evaluate PEN as a potential ligand of GPR83. Employing several second messenger and other GPCR activation assays as well as a radioligand binding assay, and using multiple GPR83 plasmids and PEN peptides from different sources, no experimental evidence was found to support a role of PEN as a GPR83 ligand.
- Subjects
RADIOLIGAND assay; G protein coupled receptors; FOOD consumption
- Publication
International Journal of Molecular Sciences, 2023, Vol 24, Issue 20, p15117
- ISSN
1661-6596
- Publication type
Article
- DOI
10.3390/ijms242015117