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- Title
Apolipoprotein (a) Impairs Endothelial Progenitor Cell-Mediated Angiogenesis.
- Authors
Wang, Ren; Zhang, Kai; Li, Shuang; Tong, Zhongyi; Li, Guohua; Zhao, Zhanzhi; Zhao, Yue; Liu, Fengtao; Lin, Xiaolong; Wang, Zuo; Jiang, Zhisheng
- Abstract
Improvement of blood flow and promotion of angiogenesis are important therapeutic measures for the treatment of ischemic peripheral vascular diseases. Since apolipoprotein (a) (apo (a)) is a glycoprotein with repetitive kringle domains exhibiting 75% to 98% structural homology with plasminogen (Plg), apo (a) may also have a negative effect on endothelial progenitor cell (EPC)-induced angiogenesis through Plg-like inhibitory effects on EPC proliferation, adhesion, migration, and angiogenesis. To evaluate the effect of apo (a) on EPCs-induced angiogenesis, EPCs were isolated from the bone marrow of apo (a) transgenic mice, wild-type litter mates, and normal mice. These cells were cultured without or with apo (a) before transplantation. Hindlimb ischemia models were surgically induced in mice, which then received an intravenous injection of 3×105 EPCs. At 3, 7, and 14 days post EPC transplantation, the adhesion, migration abilities, and capillary density in calf muscles were assessed. Results indicate that apo (a) significantly reduced the adhesion and migration abilities of EPCs. Furthermore, the tubule-like formation of EPCs on Matrigel gels was damaged. In vivo experiments showed the homing of EPCs to ischemic peripheral vascular, and the number of capillary vessels decreased significantly in apo(a) transgenic mice. This study demonstrated that apo (a) could attenuate the adhesion, migration, and homing abilities of EPCs and could impair the angiogenesis ability of EPCs. Homing of endothelial progenitor cells to ischemic peripheral vascular sites and the number of capillaries were decreased in mice transgenic for Apo (a). This gene attenuated adhesion, migration, and homing abilities of EPCs and impaired angiogenesis.
- Subjects
PERIPHERAL vascular disease treatment; APOLIPOPROTEINS; ENDOTHELIAL cells; PROGENITOR cells; NEOVASCULARIZATION; BLOOD flow; CELL proliferation; LABORATORY mice
- Publication
DNA & Cell Biology, 2013, Vol 32, Issue 5, p243
- ISSN
1044-5498
- Publication type
Article
- DOI
10.1089/dna.2013.1963