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- Title
Extraction, Characterization, Antioxidant, and Immunostimulatory Activities of Polysaccharides from Hedyotis corymbosa.
- Authors
Lin, Li-mei; Xiong, Su-hui; Zhao, Ling-jia; Tang, Jie; Zhang, Zhi-min; Li, Ya-mei; Zheng, Tao; Xia, Bo-hou
- Abstract
In this study, optimization of enzyme-assisted extraction, purification, characterization, and its bioactivities of polysaccharides from Hedyotis corymbosa (HCP) was investigated. It was found that the optimum extraction conditions were 3% of enzyme concentration (X1), 30 of liquid-to-solid ratio (X2), 56°C of extraction temperature (X3), 200W of ultrasonic power (X4), 10 min of extraction time (X5), and 5 of pH value (X6). Under optimum conditions, the experimental yield (4.10 ± 0.16%) was closed to the predicted value (4.02%). The crude HCP was further purified using DEAE-52 and Sephadex G-150 gel column, and a major polysaccharide fraction from HCP, designed as HCP-1a with molecular weight of 33.9 kDa, was obtained. The HCP and HCP-1a were characterized by chemical analysis, FT-IR, and HPLC. For antioxidant activities in vitro, HCP possessed strong hydroxyl radical scavenging, DPPH radical scavenging, and Fe2+ chelating activities. In subsequent immunostimulatory studies, significantly decreased NO, IL-1β, and TNF-α concentrations were observed in both of HCP and HCP-1a treated RAW264.7 cells. Therefore, this study may indicate some insights into the application of polysaccharides from Hedyotis corymbosa as potential natural antioxidants and immunostimulants.
- Subjects
NITRIC oxide analysis; CELL lines; DEXTRAN; ENZYMES; HIGH performance liquid chromatography; HYDROGEN-ion concentration; IMMUNE system; INFRARED spectroscopy; INTERLEUKIN-1; MEDICINAL plants; POLYSACCHARIDES; TUMOR necrosis factors; ULTRASONICS; PLANT extracts; OXIDATIVE stress; CHELATING agents; IN vitro studies
- Publication
Evidence-based Complementary & Alternative Medicine (eCAM), 2018, p1
- ISSN
1741-427X
- Publication type
Article
- DOI
10.1155/2018/8617070