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- Title
Loop-Mediated Isothermal Amplification Assay Targeting the MOMP Gene for Rapid Detection of Chlamydia psittaci Abortus Strain.
- Authors
Guo-Zhen Lin; Fu-Ying Zheng; Ji-Zhang Zhou; Guang-Hua Wang; Xiao-An Cao; Xiao-Wei Gong; Chang-Qing Qiu
- Abstract
For rapid detection of the Chlamydia psittaci abortus strain, a loop-mediated isothermal amplification (LAMP) assay was developed and evaluated in this study. The primers for the LAMP assay were designed on the basis of the main outer membrane protein (MOMP) gene sequence of C. psittaci. Analysis showed that the assay could detect the abortus strain of C. psittaci with adequate specificity. The sensitivity of the test was the same as that of the nested-conventional PCR and higher than that of chick embryo isolation. Testing of 153 samples indicated that the LAMP assay could detect the genome of the C. psittaci abortus strain effectively in clinical samples. This assay is a useful tool for rapid diagnosis of C. psittaci infection in sheep, swine and cattle.
- Subjects
CHLAMYDIA psittaci; DETECTION of microorganisms; GENE amplification; MEMBRANE proteins; BACTERIAL diseases in animals; POLYMERASE chain reaction; BACTERIAL genes
- Publication
Pakistan Veterinary Journal, 2012, Vol 32, Issue 2, p273
- ISSN
0253-8318
- Publication type
Article