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- Title
High density lipoprotein inhibits the activation of sterol regulatory element-binding protein-1 in cultured cells
- Authors
Yoshida, Masaki; Harada, Nagakatsu; Yoshida, Keiko; Nakagawa, Tadahiko; Shimohata, Takaaki; Mawatari, Kazuaki; Takahashi, Akira; Sakaue, Hiroshi; Nakaya, Yutaka
- Abstract
Abstract: A link between cellular uptake of high density lipoprotein (HDL) and regulation of sterol regulatory element-binding protein-1 (SREBP-1) was investigated in vitro. HDL decreased nuclear SREBP-1 levels as well as SREBP-1 target gene expression in HepG2 and HEK293 cells. However, HDL did not repress an exogenously expressed, constitutively active form of SREBP-1. HDL increased cellular cholesterol levels, and cellular cholesterol depletion by methyl-β-cyclodextrin abolished the effects of HDL. These results suggest that HDL inhibits the activation of SREBP-1 through a cholesterol-dependent mechanism, which may play an important role in regulating lipid synthetic pathways mediated by SREBP-1.
- Subjects
HIGH density lipoproteins; STEROLS; CARRIER proteins; CELL culture; GENE expression; GENE targeting; CYCLODEXTRINS; FATTY acid synthesis
- Publication
FEBS Letters, 2010, Vol 584, Issue 6, p1217
- ISSN
0014-5793
- Publication type
Article
- DOI
10.1016/j.febslet.2010.02.034