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- Title
Gene expression profile analysis of regenerating liver after portal vein ligation in rats by a cDNA microarray system.
- Authors
Nagano, Y.; Nagahori, K.; Yoshiro, F.; Hamaguchi, Y.; Ishikawa, T.; Ichikawa, Y.; Togo, S.; Okazaki, Y.; Hayashizaki, Y.; Shimada, H.
- Abstract
Nagano Y, Nagahori K, Yoshiro F, Hamaguchi Y, Ishikawa T, Ichikawa Y, Togo S, Okazaki Y, Hayashizaki Y, Shimada H. Gene expression profile analysis of regenerating liver after portal vein ligation in rats by a cDNA microarray system. Liver International 2004: 24: –. © Blackwell Munksgaard 2004 We assessed changes in gene expression of hypertrophied liver after portal vein ligation (PL) in a test group of rats compared to a control group, which had the same size liver but no PL. The portal veins of the left and median lobes in the test group were ligated in an initial operation. Four days after the PL, the liver volume of the posterior caudate lobe (5%) increased two-fold and comprised 10% of the liver. A 90% hepatectomy was then performed, leaving only the hypertrophied posterior caudate lobe, and leaving the normal anterior and posterior caudate lobes (10%) in the control (sham) group. A comparison of the expression profiles between two groups was performed using cDNA microarrays and the hepatic ATP level was measured. The survival rate for the PL group was significantly higher than for the sham group at 4 days after the hepatectomy (56.3% and 26.7%, P<0.05). Gene expression of cyclin D1, proliferating cell nuclear antigen, cyclin A and B was upregulated, and the cyclin-dependent kinase inhibitor was downregulated. Increases were observed in: (i) pyruvate dehydrogenase, the tricarboxylic acid cycle cycle regulator, (ii) acyl-CoA dehydrogenase, the oxidation regulator, and (iii) cytochrome oxidases, the oxidative phosphorylation regulator. Hepatic ATP concentration after hepatectomy was better maintained in the PL group than in the sham group (0.48±0.01 μmol/ml vs. 0.33±0.01 μmol/ml, P<0.05). The regenerating liver increased tolerance for extended hepatectomy compared to normal liver. It is believed that this is because the induced rapid regeneration of the remaining liver after hepatectomy increases ATP metabolism.
- Subjects
LIVER regeneration; DNA microarrays; GENE expression; GENETIC regulation; LIVER surgery; HEPATECTOMY; LABORATORY rats
- Publication
Liver International, 2004, Vol 24, Issue 3, p253
- ISSN
1478-3223
- Publication type
Article
- DOI
10.1111/j.1478-3231.2004.0912.x