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- Title
Halohasta litorea gen. nov. sp. nov., and Halohasta litchfieldiae sp. nov., isolated from the Daliang aquaculture farm, China and from Deep Lake, Antarctica, respectively.
- Authors
Mou, Yun-Zhuang; Qiu, Xing-Xing; Zhao, Mei-Lin; Cui, Heng-Lin; Oh, Dickson; Dyall-Smith, Mike
- Abstract
Two halophilic archaeal strains, R30 and tADL, were isolated from an aquaculture farm in Dailing, China, and from Deep Lake, Antarctica, respectively. Both have rod-shaped cells that lyse in distilled water, stain Gram-negative and form red-pigmented colonies. They are neutrophilic, require >120 g/l NaCl and 48-67 g/l MgCl for growth but differ in their optimum growth temperatures (30 °C, tADL vs. 40 °C, R30). The major polar lipids were typical for members of the Archaea but also included a major glycolipid chromatographically identical to sulfated mannosyl glucosyl diether (S-DGD-1). The 16S rRNA gene sequences of the two strains are 97.4 % identical, show most similarity to genes of the family Halobacteriaceae, and cluster together as a distinct clade in phylogenetic tree reconstructions. The rpoB′ gene similarity between strains R30 and tADL is 92.9 % and less to other halobacteria. Their DNA G + C contents are 62.4-62.9 mol % but DNA-DNA hybridization gives a relatedness of only 44 %. Based on phenotypic, chemotaxonomic and phylogenetic properties, we describe two new species of a novel genus, represented by strain R30 (= CGMCC 1.10593 = JCM 17270) and strain tADL (= JCM 15066 = DSMZ 22187) for which we propose the names Halohasta litorea gen. nov., sp. nov. and Halohasta litchfieldiae sp. nov., respectively.
- Subjects
HALOPHILA; NUCLEIC acids; HYDROCHARITACEAE; HEREDITY; NUCLEIC acid hybridization
- Publication
Extremophiles, 2012, Vol 16, Issue 6, p895
- ISSN
1431-0651
- Publication type
Article
- DOI
10.1007/s00792-012-0485-5