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- Title
Carcinogen 4-Nitroquinoline Oxide (4-NQO) Induces Oncostatin-M (OSM) in Esophageal Cells.
- Authors
MUKHERJEE, AMITAVA; EPPERLY, MICHAEL W.; FISHER, RENEE; SHIELDS, DONNA; WEN HOU; PENNATHUR, ARJUN; LUKETICH, JAMES; HONG WANG; GREENBERGER, JOEL S.
- Abstract
Background/Aim: The earliest cellular and molecular biologic changes in the esophagus that lead to esophageal cancer were evaluated in a mouse model. We correlated numbers of senescent cells with the levels of expression of potentially carcinogenic genes in sorted side population (SP) cells containing esophageal stem cells and non-stem cells in the non-side population cells in the 4-nitroquinolone oxide (NQO)-treated esophagus. Materials and Methods: We compared stem cells with non-stem cells from the esophagus of mice treated with the chemical carcinogen 4-NQO (100 µg/ml) in drinking water. We also compared gene expression in human esophagus samples treated with 4-NQO (100 µg/ml media) to non-treated samples. We separated and quantitated the relative levels of expression of RNA using RNAseq analysis. We identified senescent cells by luciferase imaging of p16+/LUC mice and senescent cells in excised esophagus from tdTOMp16+ mice. Results: A significant increase in the levels of RNA for oncostatin-M was found in senescent cells of the esophagus from 4-NQO-treated mice and human esophagus in vitro. Conclusion: Induction of OSM in chemically-induced esophageal cancer in mice correlates with the appearance of senescent cells.
- Subjects
CARCINOGENS; NITROQUINOLINE oxide; ONCOSTATIN M; ESOPHAGEAL cancer; RNA sequencing
- Publication
In Vivo, 2023, Vol 37, Issue 2, p506
- ISSN
0258-851X
- Publication type
Article
- DOI
10.21873/invivo.13108