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- Title
Potencialidades de bacterias productoras de polihidroxialcanoatos (PHA) aisladas de Asparagus officinalis L.
- Authors
Altamirano, Luz; Ramos, Edwin; Iglesias-Osores, Sebastián; Carreño-Farfán, Carmen
- Abstract
The objective was to determine the production of polyhydroxyalkanoates produced by potentially nitrogen-fixing bacteria isolated from Asparagus officinalis L., in 2017. The method used was through samples of previously disinfected rhizospheric soil, roots, and stems, they were cultured in nitrogen-free media to isolate rhizospheric and endophytic fixing bacteria, respectively. The production of PHA granules by these bacteria was studied in fermentation broth with glycerol as a carbon source, coloring them with Sudan Black B after 24 and 48 h. The two bacterial cultures in which the largest number of cells with PHA granules was observed were fermented with glycerol in the balanced and unbalanced media for 36 h, determining the biomass. Some of the results were 51.1% of the fixing bacteria were rhizospheric and 48.9% endophytic. PHA granules were detected in 56.67% of the bacteria. The Yp/x yield was 0.57855 g g-1 (Azospirillum sp.155) and 0.22543 g g-1 (Azospirillum sp. 130) in the balanced medium and 0.76718 g g-1 (Azospirillum sp. 155) and 0.26229 g g-1 (Azospirillum sp. 130) in the unbalanced medium, after 32 h of fermentation. In conclusion, the production of PHAs by nitrogen-fixing bacteria isolated from asparagus was demonstrated.
- Subjects
ASPARAGUS; ENDOPHYTIC bacteria; NITROGEN-fixing bacteria; AZOSPIRILLUM; BACTERIAL cultures
- Publication
Revista Mexicana de Ciencias Agrícolas, 2022, Vol 13, Issue 8, p1349
- ISSN
2007-0934
- Publication type
Article
- DOI
10.29312/remexca.v13i8.2931