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- Title
饲粮中性洗涤纤维水平对黑藏羊肌纤维类型组成比例与肉质特性的影响.
- Authors
周力; 王志有; 杨葆春; 侯生珍; 张峰硕; 桂林生
- Abstract
The effects of different neutral detergent fiber (NDF) diets on body size indexes, muscle fiber type and meat quality of Qinghai black Tibetan sheep were investigated. Forty healthy 2-month-old black Tibetan sheep with similar body weights [(10.28±0.43 kg] were randomly divided into 2 groups with 20 rams per group. They were fed diets with differing neutral detergent fiber (NDF) levels of 26.33% (group L) and 46.14% (group H). Using enzyme-linked immunosorbent assay, ATPase histochemical stain and real-time PCR, the muscle fiber characteristics, gene expression of myosin heavy chains (MyHCs) and meat quality in Black Tibetan sheep were evaluated. Results were as follows: 1) The tagma indexes in group H were significantly lower than those in group L (P<0.05). 2) The number of type IIa muscle fibers in group H was significantly more than in group L (P<0.05), and the areas of type I and type IIa muscle fibers in group H were also greater than in group L (P<0.05).3) The mRNA expression levels of MyHC I and MyHC IIa in group L were lower than those in group H (P<0.05), but the mRNA expression levels of MyHC IIb and MyHC IIx exhibited an opposite movement (P<0.05). 4) Compared with group L, superoxide dismutase, glutathione peroxidase, catalase and total antioxidant capacity contents increased (P>0.05) in group H, while the malondialdehyde (MDA) content was significantly decreased with in group L. 5) The muscle shear force in Group L was higher than that in Group H (P<0.05), while the redness (a*) was lower than that in Group H (P>0.05). In conclusion, compared with a low-NDF diet, the high-NDF diet effectively reduced the proportion of glycolic muscle fiber and also enhanced antioxidant capacity, improving the muscle quality of black Tibetan sheep.
- Publication
Acta Prataculturae Sinica, 2022, Vol 31, Issue 11, p75
- ISSN
1004-5759
- Publication type
Article
- DOI
10.11686/cyxb2021439