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- Title
Methylation Changes of Lysine 9 of Histone H3 during Preimplantation Mouse Development.
- Authors
Seungeun Yeo; Kyung-Kwang Lee; Yong-Mahn Han; Yong-Kook Kang
- Abstract
Immediately after fertilization, a chromatin remodeling process in the oocyte cytoplasm extracts protamine molecules from the sperm-derived DNA and loads histones onto it. We examined how the histone H3-lysine 9 methylation system is established on the remodeled sperm chromatin in mice. We found that the paternal pronucleus was not stained for dimethylated H3-K9 (H3-m2K9) during pronucleus development, while the maternal genome stained intensively. Such H3-m2K9 asymmetry between the parental pronuclei was independent of HP1β localization and, much like DNA methylation, was preserved to the two-cell stage when the nucleus appeared to be compartmentalized for H3- m2K9. A conspicuous increase in H3-m2K9 level was observed at the four-cell stage, and then the level was maintained without a visible change up to the blastocyst stage. The behavior of H3-m2K9 was very similar, but not identical, to that of 5-methylcytosine during preimplantation development, suggesting that there is some connection between methylation of histone and of DNA in early mouse development.
- Publication
Molecules & Cells (Springer Nature), 2005, Vol 20, Issue 3, p423
- ISSN
1016-8478
- Publication type
Article
- DOI
10.1016/s1016-8478(23)13248-1