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- Title
Altered Expression of Phospholipase D1 in Spontaneously Hypertensive Rats.
- Authors
Do Sik Min; Kweon-Haeng Lee; Jong-Soo Chang; Bong-Hyun Ahn; Duck-Joo Rhie; Shin-Hee Yoon; Sang June Hahn; Myung-Suk Kim; Yang-Hyeok Jo
- Abstract
To clarify the involvement of phospholipase D (PLD) in the mechanism underlying genetically-induced hypertension, we investigated the activity and expression levels of PLD in tissues taken from spontaneously hypertensive rats (SHR), and their normotensive controls, Wistar-Kyoto rats (WKY). The ADP-ribosylation factor 3 (ARF3)-dependent PLD activity and protein levels of PLDI from SHR increased significantly in the brain and liver, but not in the heart and kidney, compared to those of WKY. The activity and expression of PLD were the same between the homogenated whole kidneys of the two strains; however, there were topographical differences in the expression and activity of PLD between the kidneys of the two strains. The activity and expression level of PLD gradually increased from the cortex to the inner medulla of WKY. The enzyme activity, and amount of PLD in the inner stripe of the outer medulla and in the inner medulla, was significantly lower in SHR than in WKY. Taken together, these results suggest that the distinctly distributed patterns of PLD in the kidney may be associated with differential signal transduction pathways that are involved in hypertension in conjunction with an increase of PLD activity in the brain and liver.
- Subjects
PHOSPHOLIPASES; KIDNEYS; HYPERTENSION; BRAIN; LIVER
- Publication
Molecules & Cells (Springer Nature), 2001, Vol 11, Issue 3, p386
- ISSN
1016-8478
- Publication type
Article
- DOI
10.1016/s1016-8478(23)17051-8