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- Title
Preliminary X-ray Diffraction Analysis of Purine Nucleoside Phosphorylase from the Haloalkaliphilic Bacterium Halomonas chromatireducens.
- Authors
Safonova, T. N.; Antipov, A. N.; Veiko, V. P.; Mordkovich, N. N.; Okorokova, N. A.; Dorovatovskii, P. V.; Polyakov, K. M.
- Abstract
Crystals of the enzyme purine nucleoside phosphorylase from the extremophilic bacterium Halomonas Chromatireducens AGD 8-3, suitable for X-ray diffraction, were grown by the vapor-diffusion method. The X-ray diffraction data were collected from these crystals at the Belok beamline of the Kurchatov synchrotron radiation source (National Research Centre "Kurchatov Institute") at 100 K to 1.80 Å resolution. The X-ray diffraction data were processed in the space groups P1, P2, P21, and P622. The structure was solved by the molecular replacement method taking into account the twinning in the space groups P21 and P1 with one and two hexamers of the enzyme per asymmetric unit, respectively.
- Subjects
HALOMONAS (Bacteria); X-ray diffraction; PHOSPHORYLASES; SYNCHROTRON radiation sources; SPACE groups; HYDROGEN bonding
- Publication
Crystallography Reports, 2023, Vol 68, Issue 6, p920
- ISSN
1063-7745
- Publication type
Article
- DOI
10.1134/S106377452360093X