We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Alterations in DNA methylation machinery in a rat model of osteoarthritis of the hip.
- Authors
Kawarai, Yuya; Nakamura, Junichi; Hagiwara, Shigeo; Suzuki-Narita, Miyako; Inage, Kazuhide; Ohtori, Seiji
- Abstract
Background: This study aimed to validate alterations in the gene expression of DNA methylation-related enzymes and global methylation in the peripheral blood mononuclear cell (PBMC) and synovial tissues of animal hip osteoarthritis (OA) models. Methods: Animals were assigned to the control (no treatment), sham (25 µL of sterile saline), and OA (25 µL of sterile saline and 2 mg of monoiodoacetate) groups. Microcomputed tomography scan, histopathological assessment and pain threshold measurement were performed after induction. The mRNA expression of the DNA methylation machinery genes and global DNA methylation in the PBMC and hip synovial tissue were evaluated. Results: The OA group presented with hip joint OA histopathologically and radiologically and decreased pain threshold. The mRNA expression of DNA methyltransferase (Dnmt 3a), ten–eleven translocation (Tet) 1 and Tet 3 in the synovial tissue of the OA group was significantly upregulated. Global DNA methylation in the synovial tissue of the OA group was significantly higher than that of the control and sham groups. Conclusions: The intra-articular administration of monoiodoacetate induced hip joint OA and decreased pain threshold. The DNA methylation machinery in the synovial tissues of hip OA was altered.
- Subjects
ENZYME analysis; DNA methyltransferases; BIOLOGICAL models; SYNOVIAL membranes; PHYSIOLOGIC salines; ACETIC acid; RESEARCH funding; COMPUTED tomography; PAIN threshold; DESCRIPTIVE statistics; BLOOD cells; GENE expression; DNA methylation; RATS; MESSENGER RNA; ANIMAL experimentation; HIP osteoarthritis
- Publication
Journal of Orthopaedic Surgery & Research, 2024, Vol 19, p1
- ISSN
1749-799X
- Publication type
Article
- DOI
10.1186/s13018-024-04847-0