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- Title
The effect of deep eutectic solvents on the asymmetric hydrolysis of styrene oxide by mung bean epoxide hydrolases.
- Authors
Peng, Fei; Zhao, Ying; Li, Fang-Zhou; Zong, Min-Hua; Lou, Wen-Yong
- Abstract
Background: Deep eutectic solvents have attracted considerable attention in numerous fields. There is little information on mung bean epoxide hydrolase-catalyzed epoxides in deep eutectic solvent-containing system.Results: Adding deep eutectic solvents with hydrogen bond donor of acids to phosphate buffer resulted in an obvious decrease in the optical purity of product; nevertheless, the relative slight change was observed by the addition of the deep eutectic solvents with hydrogen bond donor of alcohols and urea. Of the tested deep eutectic solvents, 10% additional amount of choline chloride/trithylene glycol can cause a significant improvement in the enantiopurity of product, from 83.2 ± 1.3% to 87.9 ± 0.3%. Moreover, with the increase in the addition amount of choline chloride/triethylene glycol from 10 to 30%, the enantiomeric excess of product enhanced from 87.9 to 94%, but a decline of product yield was observed. Finally, the evaluation of enzyme stability showed that the additional amount from 10% to 30% was not beneficial to the activity recovery.Conclusion: In short, adding choline chloride/triethylene glycol contributes to the improvement in the optical purity of (<italic>R</italic>)-1-phenyl-1, 2-ethanediol in the catalysis of styrene oxide by mung bean epoxide hydrolases; meanwhile, the enzyme immobilization could be essential.<graphic></graphic>
- Subjects
SOLVENT analysis; HYDROLYSIS; STYRENE oxide; MUNG bean enzymes; HYDROLASES regulation; PHYSIOLOGY
- Publication
Bioresources & Bioprocessing, 2018, Vol 5, Issue 1, p0
- ISSN
2197-4365
- Publication type
Article
- DOI
10.1186/s40643-018-0191-y