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- Title
Introduction of an AmpR-L2 intergenic segment attenuates the induced β-lactamase activity of Stenotrophomonas maltophilia.
- Authors
Chang, Y.-C.; Huang, Y.-W.; Chiang, K.-H.; Yang, T.-C.; Chung, T.-C.
- Abstract
Expression of the L1 and L2 β-lactamase genes is generally regulated by a LysR type regulator of the AmpR in Stenotrophomonas maltophilia. The ampR gene is located immediately upstream of L2 and is transcribed divergently, forming an ampR-L2 module. The ampR-L2 modules of 16 S. maltophilia isolates were analyzed, revealing that the ampR-L2 intergenic (IG) regions show a significant genetic diversity, whereas AmpR proteins are highly conserved. The induction potential of the different AmpR toward the different ampR-L2 IG regions was evaluated by introducing the various IG- xylE transcriptional fusion constructs into a wild S. maltophilia strain. The induction levels achieved in the various AmpR-IG pairs display quantitative differences; meanwhile, the host β-lactamase activity, in some cases, is attenuated by the introduced IG segment. Similar β-lactamase attenuation phenomenon was observed in Enterobacter cloacae with an ampR-L2 IG segment of S. maltophilia. A concept of oligonucleotides attenuator for the development of an antimicrobial agent is proposed.
- Subjects
BETA lactamases; ENTEROBACTER cloacae; OLIGONUCLEOTIDES; PSEUDOMONAS aeruginosa; ANTI-infective agents
- Publication
European Journal of Clinical Microbiology & Infectious Diseases, 2010, Vol 29, Issue 7, p887
- ISSN
0934-9723
- Publication type
Report
- DOI
10.1007/s10096-010-0924-0