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- Title
Analysis of gene expression in rabbit nuclear transfer embryos: Use of single-embryo mRNA differential display.
- Authors
Wen Yong Li, William R.; Wei Dong Yu, William R.; Bing Qi, William R.; Xin He; Yu Ge Wang, William R.; Gui Sheng Liu, William R.; Miao Du; Qing Xuan Chen, William R.
- Abstract
Lack of or abnormal expression of developmentally important genes is believed to hamper early development of the nuclear transfer (NT) embryo. To identify stage-specific genes in rabbit NT embryo development, mRNA differential display was used to compare the mRNA content of rabbit NT embryos at different developmental stages, from Metaphase II oocytes to 8–16-cell stage embryos. Thirty-four zygotic transcripts, which abruptly appeared at the 8–16-cell stage in rabbit NT embryos, were isolated; 11 of these were potential novel genes with no matches in the current databases. Of the remaining 23, 12 were matched with established sequence tags with functions uncharacterized and the other 11 were homologous to those in the European Molecular Biology Laboratory (EMBL) and GenBank databases . The differential expression of eight of the 34 amplicons were confirmed by reverse Northern blotting, and four positive clones were validated. Previous studies and present data indicated that these three genes were probably related to preimplantation rabbit embryo development.
- Subjects
GENES; EMBRYO transfer; MESSENGER RNA; ZYGOTES; CELLS
- Publication
Development, Growth & Differentiation, 2003, Vol 45, Issue 5/6, p543
- ISSN
0012-1592
- Publication type
Article
- DOI
10.1111/j.1440-169X.2003.00720.x