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- Title
RCL2, a potential formalin substitute for tissue fixation in routine pathological specimens.
- Authors
Masir, Noraidah; Ghoddoosi, Mahdiieh; Mansor, Suhada; Abdul-Rahman, Faridah; Florence, Chandramaya S.; Mohamed-Ismail, Nor Azlin; Tamby, Mohammad-Rafaee; Md-Latar, Nani Harlina
- Abstract
Masir N, Ghoddoosi M, Mansor S, Abdul-Rahman F, Florence C S, Mohamed-Ismail N A, Tamby M-R & Md-Latar N H (2012) Histopathology 60, 804-815 RCL2, a potential formalin substitute for tissue fixation in routine pathological specimens Aims: To investigate RCL2 as a fixative for tissue fixation in routine histopathological examination and to assess tissue suitability for ancillary investigations. Methods and results: Forty-nine samples from 36 fresh specimens were cut into three equal pieces and fixed in RCL2 diluted in 100% ethanol, RCL2 in 95% ethanol, or neutral buffered formalin as control. Suitability for microtomy, quality of histomorphology, histochemistry, immunohistochemistry, fluorescent and silver in-situ hybridization analysis and extracted genomic DNA were assessed. Microtomy was straightforward in most tissue blocks, but there was difficulty in cutting in approximately a quarter of samples, which required careful handling by an experienced technician. There were no significant differences in tissue morphology between RCL2- and formalin-fixed tissues ( P = 0.08). Generally, the quality of histochemical staining, immunohistochemistry and in-situ hybridization were comparable to that of formalin-fixed tissues. Inconsistent immunoreactivity was noted, however, with antibodies against pan-cytokeratin and progesterone receptor. Genomic DNA concentration was higher in RCL2-fixed tissues. Using RCL2 diluted in 95% ethanol did not affect fixation quality. Conclusion: RCL2 is a potential formalin substitute suitable as a fixative for use in routine histopathological examination; however, difficulty in microtomy and occasional discrepancies in immunohistochemical reactivity require further optimization of the methodology.
- Subjects
FORMALDEHYDE analysis; TISSUE fixation (Histology); DIAGNOSTIC specimens; HISTOPATHOLOGY; MICROTOMY; DIAGNOSTIC immunohistochemistry; DIAGNOSTIC use of in-situ hybridization
- Publication
Histopathology, 2012, Vol 60, Issue 5, p804
- ISSN
0309-0167
- Publication type
Article
- DOI
10.1111/j.1365-2559.2011.04127.x