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- Title
Molecular tagging of a senescence gene by introgression mapping of a stay-green mutation from Festuca pratensis.
- Authors
Moore, B. J.; Donnison, I. S.; Harper, J. A.; Armstead, I. P.; King, J.; Thomas, H.; Jones, R. N.; Jones, T. H.; Thomas, H. M.; Morgan, W. G.; Thomas, A.; Ougham, H. J.; Huang, L.; Fentem, T.; Roberts, L. A.; King, I. P.
- Abstract
• Intergeneric hybrids betweenLolium multiflorumand Festuca pratensis (Lm/Fp) and their derivatives exhibit a unique combination of genetic and cytogenetic characteristics: chromosomes undergo a high frequency of homoeologous recombination at meiosis; the chromosomes of the two species can easily be discriminated by genomicin situhybridization (GISH); recombination occurs along the entire length of homoeologous bivalents; a high frequency of marker polymorphism is observed between the two species.• This combination of characters has been used to transfer and isolate aF. pratensischromosome segment carrying a mutant‘stay-green’ gene conferring a disrupted leaf senescence phenotype intoL. multiflorum.• The genetic location within the introgressedF. pratensissegment of the senescence gene has been mapped using amplified fragment length polymorphisms (AFLPs), andF. pratensis-specific AFLP markers closely flanking the green gene have been cloned.• The use of these cloned sequences as markers for the stay-green locus in marker-assisted selection programmes has been tested. The potential application ofLm/Fpintrogressions as a tool for the map-based cloning of introgressedFpgenes is discussed.
- Subjects
RYEGRASSES; MEADOW fescue; CHROMOSOMES; MEIOSIS; IN situ hybridization; GENETIC polymorphisms; GENES
- Publication
New Phytologist, 2005, Vol 165, Issue 3, p801
- ISSN
0028-646X
- Publication type
Article
- DOI
10.1111/j.1469-8137.2004.01269.x